Figure 4
Global shift of insulin responsive genes in adipocytes. (A) Cumulative frequency graph of the gene expression fold change caused by sgRNA. for genes that are upregulated in response to insulin. Threshold for gene selection: logFC > 0.67 and p < 0.2. (B) Cumulative frequency graph for genes downregulated by sgRNA1. (C,D) Same as panel A and B for sgRNA.2. Kolmogorov-Smirnov test used to calculate p value between the cumulative curves. (E–J) Quantitative RT-PCR validation for metabolism genes whose response to insulin were attenuated by LncASIR repression. Unpaired t-test used to calculate the p values. P < 0.05; *p < 0.005; **p < 0.001; ***. Error bars are SEM. Graphs have been drawn using Prism 8.
