Figure 7
DYRK1A depletion sensitizes cells to DNA damage. Clonogenic survival assay of DYRK1A-depleted cells in response to IR. U2OS cells infected with lentiviruses expressing either non-target shRNA (shControl) or two different shRNAs against DYRK1A were exposed to different IR doses, and the cell’s ability to form colonies was measured after 2 weeks of growth. DYRK1A depletion was assessed in WBs of total cell extracts from parallel samples (a representative experiment is shown). The data correspond to the mean ± SEM (shControl n = 7, shDYRK1A.1 n = 6, shDYRK1A.3 n = 3): *p < 0.05; ***p < 0.001 two-tailed Student’s t-test in pairwise comparisons. The Y-axis is represented on a logarithmic scale.
