Figure 4

Maf-deficient T_reg fail to prevent colitis in vivo. CD4⁺CD45RB^hiCD25^lo cells (0.5 × 10⁶) were intravenously injected alone or in combination with CD4⁺CD45RB^loCD25^hi cells (0.4 × 10⁶) into males and females Rag1^−/− mice from 10 to 15 weeks age. (A) Body weight curve of Rag1^−/− mice injected with either WT (upper) or Maf^ΔTcells naïve CD4⁺ T cells (bottom) alone or in combination with WT or Maf^ΔTcells T_reg. Statistical significances were calculated by a Bonferroni test after a significant two-way ANOVA. (B) Haematoxylin and eosin-stained sections of colons (Upper picture: scale bar, 200 µm. Bottom pictures: scale bar, 100 µm) and (C) Histological scores (from 0 to 6) for microscopic changes in the colons of indicated groups of mice. (D) Absolute cell numbers of total FOXP3⁺ T cells in the colons of the indicated groups of mice (n > 5 per group). (E) Percentage of MAF⁺ cells among FOXP3⁺ T cells in the colons of the indicated groups of mice (n > 5 per group). (A,C,D,E) All results are representative of 2 independent experiments with at least 2 mice per group. (C–E), Each symbol represents an individual mouse. All graphs indicate means. Error bars display SEM.