Figure 5
From: Maf deficiency in T cells dysregulates Treg - TH17 balance leading to spontaneous colitis

MafΔTreg mice do not develop colitis. (A) Representative contour plot of the expression of FOXP3 and RORγt in CD4+ T cells from spleens, mLN and colons of Maff/f and MafΔTreg mice. (B) Percentages of RORγt- Treg, RORγt+ Treg and TH17 cells from spleens, mLN and colons of Maff/f and MafΔTreg mice (n > 8 per group). (C) Percentage of MAF+ cells among RORγt- Treg, RORγt+ Treg and TH17 cells from mLN of Maff/f and MafΔTcells (n > 10 per group). (D) Haematoxylin and eosin-stained sections of colons from Maff/f and MafΔTreg mice (Upper picture: scale bar, 200 µm; bottom pictures: scale bar, 100 µm). (E) Histological scores (from 0 to 6) of microscopic changes in the colon from Maff/f and MafΔTcells mice at the age of 6 to 10 months (n = 5 per group). (F) Body weight of Maff/f and MafΔTreg male mice of 10 and 22 weeks old (n > 6 per group). (G) Total cell numbers in mLN (left) and colons (right) of Maff/f and MafΔTreg mice (n > 10 per group). (H) Absolute cell numbers of CD4+ and CD8+ T cells in colons of Maff/f and MafΔTreg mice (n > 10 per group). (B,C,E,H) Each symbol represents an individual mouse. (B,C,G,H) Data are representative of at least 2 independent experiments with at least 4 mice per group. All mice were over 20 weeks old. All graphs indicate means.