Figure 4

Sensitivity of the LB method coupled with the fixation step, when using PVDF membranes. Indicated numerals are amounts (3.0, 1.5, 0.7, 0.3, and 0.1 μg) of the pooled serum proteins were subjected to 10% SDS-PAGE. (a) The blotted membranes were treated using the traditional (top panel) or optimised fixation protocol (bottom panel). (b) Quantification of band intensities were statistically analyzed (n = 3 individual experiments). Solid bar, no fixation; White bar, sample fixation. The exposure times were the same for the same lectin blotting using fixation or no fixation. Band intensities were analysed and compared using Image Lab software (Bio-Rad Laboratories) and GraphPad Prism version 6. ^*Significantly different p < 0.05, ^**p < 0.01, ^***p < 0.001, ^****p < 0.0001. All values are means ± S.E. (error bars).