Table 1 Hybridization probes used in the present study.

From: A high-throughput and ultrasensitive identification methodology for unauthorized GMP component based on suspension array and logical calculator

Target

Sequence (5′-3′)

Tma

P35S

GGGATGACGCACAATCCCACTATCC

65.21

TNOS

GGTCTTGCGATGATTATCATATAATTTCTGTTGAATT

64.94

T35S

CCCTAATTTCCCTTATCGGGAAACTACTCAC

64.68

PAT

TGGCCGCGGTTTGTGATATCGTTAA

65.16

Rbc

ATAAGATTCATGGAATTATCTTCCACGTGGC

64.74

E9

GGTTTTCGCTATCGAACTGTGAAATGGAAATG

65.37

PINII

AGTGATTAGCATGTCACTATGTGTGCATCC

65.21

40278

CGTAGCTAACCTTCATTGTATTCCGCTTCA

65.16

305423

TGACACAAATGATTTTCATACAAAAGTCGAGAAA

64.83

CV127

AGCTGTCCCATGCCCATCAAAGAAG

65.34

MON810

CGACCTGAACGAGGACTTTCGGTAG

64.76

NK603

CCGCGTTAACAAGCTTACTCGAGGT

65.22

Bt11

GGCGGCTTATCTGTCTCAGGGG

64.70

MON88017

TTGCCGGAGTATGACGGTGACG

64.81

GA21

GCAGGTGGGTCCGGGTCG

65.09

MON87427

CGGTCGGGTCAAATGTAGAAAATCGG

65.19

MIR604

AGAAGGCGGGAAACGACAATCTGAT

64.56

UP

TTTGGTCGTGGTGGTGGTTT

60.32

  1. aThe Tm value for each hybridization probe was calculated through the NCBI primer Blast website (https://blast.ncbi.nlm.nih.gov/Blast.cgi).
Back to article page