Table 2 Practical evaluation of the LI-US system.

Variety	Matrix	GMP component		Evaluation results by LI-US			Evaluation results by qPCR		Fitness
Variety	Matrix	Authorized	Unauthorized	YES gate	OR gate	AND gate	Authorized	Unauthorized	Fitness
Maize	Maize meal	1% MON810	5% GA21	TRUE	TRUE	FALSE	+	+	YES
			0.5% GA21	TRUE	TRUE	FALSE	+	+	YES
			0.05% GA21	TRUE	TRUE	FALSE	+	+	YES
			No GA21	TRUE	TRUE	TRUE	+	−	YES
Soybean	Soybean meal	1% GTS40-3-2	5% MON89788	TRUE	TRUE	FALSE	+	+	YES
			0.5% MON89788	TRUE	TRUE	FALSE	+	+	YES
			0.05% MON89788	TRUE	TRUE	FALSE	+	?^a	YES
			No MON89788	TRUE	TRUE	TRUE	+	−	YES
Canola	Canola seeds	1% RT73	5% RF3	TRUE	TRUE	FALSE	+	+	YES
			0.5% RF3	TRUE	TRUE	FALSE	+	+	YES
			0.05% RF3	TRUE	TRUE	FALSE	+	?	YES
			No RF3	TRUE	TRUE	TRUE	+	−	YES

^aThe groups with symbol of “?” indicated the uncertainty of the evaluation results, which have achieved the Ct values above 36.

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