Figure 4

Effects of orobol on MDI-induced 4E-BP1 signaling in 3T3-L1 preadipocytes. (A) The protein expression levels of phospho- and total-4E-BP1, but not phospho- and total-eIF4E proteins, were downregulated by orobol dose-dependently. (B) Orobol suppressed PPARγ and C/EBPα expression in 3T3-L1 preadipocytes. Arrows marked on the band for C/EBPα point to specific C/EBPα proteins. The data are representative of three independent experiments that gave similar results. Presented signals from were cropped from one continuous Western blot which is displayed as Suppl. Figure.