Figure 6
From: Evolutionary mechanism leading to the multi-cagA genotype in Helicobacter pylori
Illustration of cagA copy number in PMSS1 and G27 isogenic mutant strains. The cagA gene copy number status in PMSS1 and G27 isogenic mutant strains were detected using colony PCR; and the cagA regions were confirmed by Sanger sequencing as described in Methods. Both PMSS1 isogenic mutant strains and the G27/revertant showed no changes in the cagA region; conversely, G27/2CHA-ud and G27/2CHA-d were able to delete and duplicate the cagA gene. Primer annealing sites for both colony PCR and sequencing as well as length of PCR amplicons are indicated. Recombination events in the cagA region are shown.
