Figure 1
Culture and characterization of skin- and semen-derived somatic cells of the buffalo breeding bull. Skin-derived cells have spindle-shaped morphology; whereas, semen-derived cells have polygonal-shaped morphology and form a flat monolayer (A), both the type of cells were used to produce cloned embryos that developed to blastocysts (A). Skin-derived cells expressed vimentin, a fibroblast cell type marker; whereas, semen-derived cells expressed cytokeratin, an epithelial cell type marker, which were confirmed by mRNA expression using PCR (B) and immunofluorescence staining (C). Semen-derived epithelial cells have lower cell proliferation than that of skin-derived fibroblast cells (D). Cell images were captured at 100X total magnification; whereas, blastocyst images were captured at 40X total magnification.
