Figure 3 | Scientific Reports

Figure 3

From: Autosomal Dominant Tubulointerstitial Kidney Disease with Adult Onset due to a Novel Renin Mutation Mapping in the Mature Protein

Figure 3

Expression of L381P renin isoform induces the UPR. (a) HSPA5 and (b) XBP1s expression analysis by real-time RT-qPCR. Gene expression is normalized to HPRT1. Expression of L381P isoform in HEK cells induces expression of HSPA5 (BiP) and spliced XBP1, markers of ER stress and UPR respectively. Data are expressed as mean ± s.d. (n = 3 independent experiments) (one way ANOVA, P = 1.78 ×    10−5 and P = 1 × 10−4 for HSPA5 and spliced XBP1 respectively, followed by Tukey Honest Significant Difference (HSD) post hoc test). (c) ATF6 activation assessed through the use of a luciferase-based, ATF6 reporter construct. ATF6 activation is observed upon expression of the L381P renin isoform. Data are expressed as mean ± s.d. (n = 6 independent experiments) (one way ANOVA, P = 5.78 × 10−5, followed by Tukey HSD post hoc test). (d) ATF4 activity, assessed through the use of a luciferase-based reporter construct. No increase of luciferase activity is observed in cells expressing the L381P renin isoform compared to mock or cells expressing wild type renin. Data are expressed as mean ± s.d. (n = 6 independent experiments) (one way ANOVA, P = 0.1175).

Back to article page