Figure 4 | Scientific Reports

Figure 4

From: Autosomal Dominant Tubulointerstitial Kidney Disease with Adult Onset due to a Novel Renin Mutation Mapping in the Mature Protein

Figure 4

The L381P renin isoform does not exert a dominant negative effect on trafficking of wild type renin. (a) Western blot analysis showing Flag- and HA-tagged renin isoforms in lysate and conditioned medium of stably transfected HEK cells. GAPDH is shown as a loading control. Quantification of secreted Flag-tagged wild type isoform normalized to Flag-tagged wild type protein present in cell lysate is shown. Data are expressed as mean ± s.d. (n = 3 independent experiments). (b) Western blot analysis showing Flag- and HA-tagged renin in HEK lysates after treatment with EndoH. Full-length blots (a,b) are presented in Supplementary Fig. S3. (c) Immunofluorescence analysis showing merged picture of Flag- and HA-tagged renin in red and green respectively. Bar = 40 μm. Cellular localization of wild type renin is not affected by co-expression with ER-retained L381P isoform.

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