Figure 1 | Scientific Reports

Figure 1

From: Novel Human Anti-PD-L1 mAbs Inhibit Immune-Independent Tumor Cell Growth and PD-L1 Associated Intracellular Signalling

Figure 1

Binding curves and anti-tumor effects of PD-L1_1 mAb on breast cancer cells. The binding curves were obtained by ELISA assays of PD-L1_1 tested at increasing concentrations on MDA-MB-231 (a), SK-BR-3 (b) and JIMT-1 (c) cells. MCF-7 cells were used in parallel assays as a negative control (grey curve, panel a). The expression of PD-L1 on the indicated tumor cell lines was tested by cell ELISA with a commercial anti-PD-L1 antibody (d). Effects of PD-L1_1 on MDAMB231 (gray curve), SK-BR-3 (black curve) or MCF-7 cells(dashed line). Cells were treated for 72 h with PD-L1_1 mAb tested at increasing concentrations and cell survival was expressed as percentage of viable cells with respect to untreated cells (e). Error bars depicted means ± SD. P values for the indicated treatment relative to untreated cells, are: ***P ≤ 0.001; **P < 0.01; *P < 0.05.

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