Table 1 Primary antibodies.

From: Characterization of dystroglycan binding in adhesion of human induced pluripotent stem cells to laminin-511 E8 fragment

Antibody to

Epitope

Clone

Host/Antigen Species

Source/Reference

Application

Dystroglycan

α-DG

VIA4

Mouse/Rabbit

DSHB, Iowa city, IA

ICC, FCM

Dystroglycan

α-DG (GlcA-Xyl)

IIH6

Mouse/Rabbit

DSHB, Iowa city, IA

ICC, FCM, IB

Nanog

Nanog peptide

Rabbit/Human

ReproCell, Yokohama, Japan

ICC

SSEA-4

SSEA-4

MC813-70

Mouse/Human

Merck, Kenilworth, NJ

FCM

TRA-1-81

TRA-1-81

TRA-1-81

Mouse/Human

Merck, Kenilworth, NJ

FCM

Laminin-111

mouse EHS laminn-111

Rabbit/Mouse

Sigma-Aldrich, St. Louis, MO

IB

Utrophin

C-terminus of utrophin

8A4

Mouse/Human

DSHB, Iowa city, IA

IB

  1. IHC: immunocytochemistry; FCM, Flowcytometry; IB: immunoblotting.