Figure 1
From: Increased fatty acyl saturation of phosphatidylinositol phosphates in prostate cancer progression
Establishment and characteristics of doxycycline-induced PTEN-KD prostate cells and PIPs profiles of PTEN-KD and control prostate cells. (A) PTEN expression in HEK293T cells and various prostate cell lines. Other supporting data were shown in Supplementary Fig. 1. (B) PTEN expression and AKT activation in doxycycline-induced PTEN-KD PNT1B and PTEN-WT PNT1B cells (control). Other supporting data were shown in Supplementary Fig. 2. (C) Cell proliferation in PTEN-KD and control PNT1B cells. The MTT assay was performed at 24, 48, 72, and 96 h. Cell viability was compared with cells at 24 h. *p < 0.05. (D) Total PIPs levels in PTEN-KD and control cells (n = 6) determined using mass spectrometry. The levels are reported as concentration per 1 × 106 cells. The box-dot plot presents the levels of total PIPs in PTEN-KD and control cells. (E) PIPs profiles in PTEN-KD and control cells (n = 6) determined using mass spectrometry. The levels are reported as concentration per 1 × 106 cells. Black bars, PTEN-KD cells; white bars, control cells. *p < 0.05.
