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Figure 1

From: Aminopeptidase N-null neonatal piglets are protected from transmissible gastroenteritis virus but not porcine epidemic diarrhea virus

Figure 1

Efficient generation of APN-null pigs using a combination of CRISPR/Cas9 and somatic cell nuclear transfer technology. (A) Schematic description of APN genome structure and the targeting strategy. Exons of APN are shown as orange, and exon 2 represents the sgRNA targeting site, which is enlarged to a rectangle box. Primer 1/Primer 2 are designed for genotyping in (D). (B) Schematic overview of the production of APN-null pigs. (C) Representative APN-null pigs at day 2. (D) PCR genotyping-confirmed site-specific modification at APN in the cloned pigs. Sequencing results were shown as Table S3. Lanes 1–5 represent 5 wildtype individuals and lanes 6–10 represent 5 APN-null pigs (KO1-5), full-length gels are presented in Supplementary Fig. 3A. (E) Western blot analysis confirmed absence of APN in the APN-null pigs (n = 5). Full-length blots are presented in Supplementary Fig. 3B,C.

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