Figure 1

Integrated miRNA-mRNA interaction network confirmed critical role of hsa-miR-4756-3p in TNBC patients. (A) Upregulated mRNA and downregulated miRNA were calculated, then the miRNA and mRNA interaction were assessed by microRNA master regulator analysis (MMRA) algorithm. (B) hsa-miR-4756-3p expression level from TCGA miRNA matrix was extracted and evaluated by t-test (***p < 0.001). (C) hsa-miR-4756-3p expression level and TCGA breast cancer patients’ clinical information was extracted, then hsa-miR-4756-3p relation with patients’ overall survival rate was assessed by univariate cox regression. (D) In-house 14 TNBC patients and 3 non-TNBC patient, their primary tumor mRNA was extracted and using QPCR to detect hsa-miR-4756-3p expression level.