Figure 4
From: Structural analysis of biological targets by host:guest crystal lattice engineering
L1 constructs with bound peptides. (A) Overview of EngBF-L1-DARPin_G10:c-pep1 complex. EngBF and DARPin_G10 are shown as a cartoon in light blue and orange, respectively, and the disulfide bridge Cys1655-Cys342* as spheres. The N-terminal beta-strand of the symmetry-related complex is shown in grey. The simulated annealing difference Fourier map is shown in green and red at contour levels of +3.5σ and −3.5σ, respectively. (B) Final 2mFo − DFc map of peptide c-pep1 bound to EngBF-L1-DARPin_B6. The map was contoured at 1.1σ. (C) B-factor colouring of peptide c-pep1 (sticks) bound to EngBF-L1-DARPin_G10 (surface) with values ranging from 20 Å2 (blue) to 203 Å2 (red). Symmetry mates are shown as grey surfaces. (D) Structure of pep2 in complex with EngBF-L1-DARPin_D12. The 2mFo − DFc map was contoured at 1.0 σ.
