Figure 6 | Scientific Reports

Figure 6

From: Microbial metabolite deoxycholic acid controls Clostridium perfringens-induced chicken necrotic enteritis through attenuating inflammatory cyclooxygenase signaling

Figure 6

COX inhibitor aspirin alleviates C. perfringens-induced inflammatory response in chicken splenocytes. Splenocytes isolated from broiler chickens were infected with C. perfringens (MOI 100) for 4 hr or stimulated with chicken INFγ (1 μg/ml) for 2 hours in the presence of 1.2 mM aspirin. RNA was extracted, reverse-transcribed, and quantified using a Bio-Rad 384 PCR platform. (A) Infγ, Litaf, Mmp9, and Ptgs2 mRNA fold change normalized to Gapdh. (B) Gene expression fold change in the presence of chicken INFγ (ch-INFγ) and aspirin. All graphs depict mean ± SEM. *P < 0.05; ***P < 0.001. Results are representative of 2 independent experiments.

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