Figure 5

TPEN treatment induces fibrosarcoma death in a mice model. (a–d) Crystal violet assay reveals that 3-MA, chloroquine, and TPEN treatment lead to more severe cytotoxicity in the ZIP13−/− cell line and the HT1080 fibrosarcoma cell line, compared to the ZIP13+/+ cell line, as well as normal human dermal fibroblast. The data are presented as mean ± SD of three independent experiments (*P < 0.05). (e) Flowchart of HT1080 fibrosarcoma xenograft model. (f) Tumor image representative of 5 independent experiments. (g,h) Tumor volume and weight were significantly decreased by TPEN treatment compared to the control (*P < 0.05). (i) IHC analysis showed that TPEN treatment decreased LC3 and increased the level of cleaved caspase 3, compared to the control in tumor tissue. Scale bars = 500 μm.