Figure 4
From: Identification and Validation of a Novel Biologics Target in Triple Negative Breast Cancer
GABRP protein detection by IF and IHC in breast cancer cell lines and breast tumors. (A) IF detection of GABRP (green) in MDA-MB-468 (left) and SK-BR3 (right) showed membrane and cytoplasmic localization of the protein. Magnification 20×. Cell nuclei were counter-stained with Hoechst (blue) dye. (B) GABRP detection by chromogenic IHC in FFPE TNBC cell pellets (MDA-MB-468, left) and (SK-BR3, right) from the Yale breast cancer index tissue microarray (YTMA279) showing cytoplasmic and membranous localization of the protein. (C) Representative images from IHC chromogenic detection of GABRP in breast cancer TMAs. GABRP-positive case (left) with signal in tumor epithelial cells and a GABRP-negative case (right). Magnification 40X. (D) Distribution of GABRP-positive and negative cases in the breast cancer TMA stained by IHC. Minimum of 100 tumor epithelial cells within each case were examined and positivity cut-off was set at 1%. (E) Comparison of the percentage of GABRP positive cells in TNBC and non-TNBC tumors in the cases scored positive from breast cancer TMAs.
