Table 3 The primer sequences of miRNA. 5 s rRNA was universal adaptor primer which was used for normalizing the expression of miRNA.
miRNA | Sequence | Amplification fragment size | Annealing temperature (°C) |
|---|---|---|---|
cgr-miR-130b-3p | AGTGCAATGATGAAAGGGCAT | 75 | 60 |
cgr-miR-142-5p | GCCCATAAAGTAGAAAGCACTACAA | 77 | 60 |
cgr-miR-34c-3p | AATCACTAACCACACGGCCA | 74 | 60 |
cgr-miR-21-3p | CAACAGCAGTCGATGGGCT | 73 | 60 |
cgr-miR-504 | AGACCCTGGTCTGCACCTCTA | 75 | 60 |
Novel_118 | GCTAACACTGTCTGGTAACGATGTA | 78 | 60 |
Novel_117 | CCCGGTTTATGTATGTGTATATGTATAAA | 80 | 60 |
Novel_135 | GGCTAGAAAGAGGCTGGGGAT | 75 | 60 |
5s rRNA | / | 72 | 60 |
