Figure 2 | Scientific Reports

Figure 2

From: PRAS40 suppresses atherogenesis through inhibition of mTORC1-dependent pro-inflammatory signaling in endothelial cells

Figure 2

PRAS40 knockdown promotes pro-inflammatory signaling in endothelial cells. (a) Representative immunoblot for indicated proteins after siRNA-mediated knockdown of PRAS40 in HUVECs and treatment with TNFα (2,5 ng/ml). (b) Statistical analysis for indicated proteins based on analysis of 3 individual biological replicates. Data represent mean ± SEM; *P < 0.05; ***P < 0.001; ****P < 0.0001 (ANOVA followed by Bonferroni’s post-hoc comparisons). (c) Quantitative RT-PCR analysis for indicated transcripts after siRNA-mediated knockdown of PRAS40 in HUVECs and treatment with TNFα (2,5 ng/ml) based on analysis of 3 individual biological replicates. Data represent mean ± SEM; *P < 0.05; ***P < 0.001; ****P < 0.0001 (ANOVA followed by Bonferroni’s post-hoc comparisons).

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