Figure 5
Pharmacological mTORC1-inhibition mimicks effects of PRAS40 overexpression in endothelial cells. (a) Representative immunoblot for indicated proteins after pharmacological mTORC1-inhibition with torin1 upon induction with indicated TNFα concentrations and timepoints. (b,c) Statistical analysis for indicated proteins based on analysis of 2 individual biological replicates. Data represent mean ± SEM; **P < 0.01; ***P < 0.001; ****P < 0.0001 (ANOVA followed by Bonferroni’s post-hoc comparisons). (c) Statistical analysis of fold changes in adhesion of mononcytes on HUVECs upon induction with TNFα and after treatment with torin1. Data represent mean ± SEM; ****P < 0.0001 (ANOVA followed by Bonferroni’s post-hoc comparisons). (e) Analysis of apoptotic HUVECs measured by flow-cytometry (annexin positive cells) after torin1 treatment and induction with TNFα (2,5 ng/ml) for 24 h based on the analysis of 3 individual biological replicates. Data represent mean ± SEM; **P < 0.01; ****P ≤ 0.0001 (ANOVA followed by Bonferroni’s post-hoc comparisons).
