Figure 4
RTD-1 concentration-time profiles following i.v. and i.p. administration. (a) Single. dose PK. Single doses of RTD-1 (5 mg/kg) were administered i.v. or i.p. at T = 0 as described in Methods. Blood was collected at the indicated time points and plasma RTD-1 levels were quantified by LC-MS/MS. Mean plasma levels and SD are plotted at each time point. (b) Multiple dose administration of RTD-1. Mice received 1-7 doses of 5 mg/kg of RTD-1 and plasma levels were determined by LC-MS/MS and plotted versus time and number of injections (Methods). The plot shows the 1 h (n = 12) and 4 h (n = 11) plasma levels of mice receiving 1 injection i.p. at T = 0. All other values are plasma levels (mean +/− S.D.) obtained 1 h after additional daily i.p. doses administered for up to 6 days with times of collection by group: 25 h, n = 4; 73 h, n = 8; 121 h, n = 4; 145 h, n = 7.
