Figure 6
RTD-1 promotes fungal clearance and restores immune homeostasis in long term survivors. (a) RTD-1 mediated fungal clearance. Fungal burdens were quantified in homogenates of individual kidneys of long term survivors treated i.p. at T = 0 with saline, RTD-1 or Fluco (mice from studies presented in Fig. 3a,b). Saline-treated moribund mice (M) were euthanized between 5 and 10 d p.i. CFU/g (geometric means with 95% CI) were plotted for each treatment cohort and analyzed by Fisher’s LSD test. (b) RTD-1 normalizes blood neutrophils and monocytes in long term survivors of systemic candidiasis. Blood PMNs and monocytes were quantified in naÏve mice, moribund saline treated mice, and RTD-1 treated long term survivors (n = 6 per cohort; mice from studies presented in Fig. 3a,b). Effect of RTD-1 treatment was analyzed by Mann-Whitney test. (c) Cytokines normalized by RTD-1 in candidemic long term survivors. In long term survivors, RTD-1 treatment restored pro-inflammatory biomarkers TNF-α, IL-10, and IL-6 to levels equivalent to those of naÏve animals (n = 3-11 in each cohort). Effect of RTD-1 treatment was analyzed by Mann-Whitney test.
