Figure 2
From: Paeonol attenuates inflammation by targeting HMGB1 through upregulating miR-339-5p
HMGB1 was a direct target of miR-339-5p. (A) The target sites of miR-339-5p in the HMGB1 sequence were predicted by bioinformatic software. (B) The construction profile of the pLUC-HMGB1 vector, which contained the miR-339-5p target sites in the HMGB1 3′-UTR, is presented in the diagram. (C,D) The relative luciferase activity in RAW264.7 cells was measured after the co-transfection of the miR-339-5p mimic, mimic NC, inhibitor or inhibitor NC with HMGB1-WT-3′-UTR or HMGB1-MUT-3′-UTR. (E) The expression of the target protein HMGB1 in RAW264.7 cells was analysed by Western blotting. (F) The expression of the target protein HMGB1 in RAW264.7 cells was analysed by immunofluorescence staining (magnification, 400×). Note: *P < 0.05, ***P < 0.001.
