Table 1 Primer sequences, annealing temperature and product size for MSP of target genes.
Genes | Forward primer (5′ → 3′) | Annealing temperature(oC) | Product size (bp) | |
|---|---|---|---|---|
CDKN2A | M | F:TTATTAGAGGGTGGGGCGGATCGC | 62 | 150 |
R:GACCCCGAACCGCGACCGTAA | ||||
U | F:TTATTAGAGGGTGGGGTGGATTGT | 62 | 151 | |
R:CAACCCCAAACCACAACCATAA | ||||
hMLH1 | M | F:ACGTAGACGTTTTATTAGGGTCGC | 60 | 118 |
R:CCTCATCGTAACTACCCGCG | ||||
U | F:TTTTGATGTAGATGTTTTATTAGGGTTGT | 60 | 124 | |
R:ACCACCTCATCATAACTACCCACA | ||||
MGMT | M | F:TTTCGACGTTCGTAGGTTTTCGC | 53 | 81 |
R:GCACTCTTCCGAAAACGAAACG | ||||
U | F:TTTGTGTTTTGATGTTTGTAGGTTTTTGT | 53 | 93 | |
R:AACTCCACACTCTTCCAAAAACAAAACA | ||||
CSF2 | M | F: TGATTATTTAGGGAAAAGGTTTATC | 56 | 105 |
R: ATAACCACAAAATACCAAAAAAACG | ||||
U | F: ATTATTTAGGGAAAAGGTTTATTGT | 60 | 104 | |
R: AATAACCACAAAATACCAAAAAAACA | ||||
DIS3L2 | M | F: GTCGTAGTTGAATCGTCGATTAC | 54 | 134 |
R: TTACTAAAAAAAATACTCTTCCGAA | ||||
U | F: GTTGTAGTTGAATTGTTGATTATGA | 55 | 134 | |
R: TTACTAAAAAAAATACTCTTCCAAA | ||||
