Figure 1
From: Extracellular NAD+ enhances PARP-dependent DNA repair capacity independently of CD73 activity
Effect of NAD+ depletion on DNA damage and repair in MCF-7 cells as determined using the CometChip assay62. (A) Level of DNA damage in MCF-7 cells exposed (1 hr) to different concentrations of various DNA damaging agents. (B) Level of intracellular NAD+ in MCF-7 cells 24 hrs after treatment with FK866 (30 nM) as compared to control. Statistical analysis was conducted via an unpaired t-test (****p < 0.001). (C) Level of DNA damage and repair capacity in MCF-7 cells exposed for one hr to etoposide (10 μM) in the presence or absence of FK866 (FK; treatment with FK866 results in a 70–90% decrease in total cellular NAD+ levels16). (D) Level of DNA damage and DNA repair capacity in MCF-7 cells exposed for one hr to different MMS concentrations (0, 0.125, 0.25, 0.5 and 1 mM) in the presence or absence of FK866 (FK; treatment with FK866 results in a 70–90% decrease in total cellular NAD+ levels16). Data is expressed as % Tail DNA, with standard deviation. Plots are the average of 8–12 wells from two-three independent CometChips; >1000 comets per bar. Statistical analysis was performed using GraphPad Prism 7 and two-way ANOVA followed by post-hoc with Tukey’s multiple comparison test (**p < 0.0029, ***<0.0008, ****<0.0001).
