Figure 2 | Scientific Reports

Figure 2

From: Genome-wide screening of lipoproteins in Actinobacillus pleuropneumoniae identifies three antigens that confer protection against virulent challenge

Figure 2

Immunoreactivity of soluble lipoproteins. Protein samples were separated by 12% SDS-PAGE and blotted onto nitrocellulose membranes. To test the serovar-cross reactivity of these lipoproteins cloned from A. pleuropneumoniae serovar 3, the membranes were incubated with rabbit polyclonal antibodies against A. pleuropneumoniae serovar 7, and Dylight-800-conjugated goat anti-rabbit IgG, sequentially, and images were viewed using a scanned infrared imaging system (Odyssey; LICOR). A white arrow indicates a specific signal for lipoprotein. The lane number indicates the protein number of A. pleuropneumoniae JL03.

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