Figure 10
From: Structure and mechanism of a redesigned multidrug transporter from the Major Facilitator Superfamily
Structural comparison between E26T/D34M/A150E and Q131R. (a) Overlay of the Cm-bound structure of E26T/D34M/A150E at pH 8.0 (with the N and C domains colored cyan and yellow, respectively; Cm colored in light pink) and that of MdfA variant Q131R (PDB 4ZOW, colored gray), with the substrate-binding amino acids and Cm drawn as stick models. (b) Overlay of the DXC-bound structure of E26T/D34M/A150E (same color scheme as in a; DXC colored green) and that of Q131R (PDB 4ZP0, colored gray), with the substrate-binding amino acids and DXC drawn as stick models. (c) Overlay of the LDAO-bound structure of E26T/D34M/A150E (same color scheme as in a; LDAO colored magenta) and that of Q131R (PDB 4ZP2, colored gray), with the substrate-binding amino acids and LDAO drawn as stick models. (d) Overlay of the LDAO-bound structure of E26T/D34M/A150E (same color scheme as in c) and that of Q131R (PDB 4ZP2, colored gray) to highlight the protonation sites, with the relevant amino acids and LDAO drawn as stick models. This figure is prepared with the software PyMOL, version 2.3.2, http://pymol.org.
