Figure 5
From: Structure and mechanism of a redesigned multidrug transporter from the Major Facilitator Superfamily
Deoxycholate (DXC) and chloramphenicol (Cm) resistance assays. Bacteria expressing the E26T/D34M/A150E variants were tested for DXC and/or Cm resistance in solid media. Five consecutive 10-fold dilutions of bacteria were prepared, and 4 µl of each dilution were plated on plates containing kanamycin, IPTG, in addition to 300 µg/ml DXC, 0.5 µg/ml Cm, or both 300 µg/ml DXC and 0.5 µg/ml Cm. The ability of bacteria to form single colonies was visualized after overnight incubation. The height of the bars corresponds to the maximal dilution at which bacterial growth was observed. Three different transformants were examined for each E26T/D34M/A150E variant. Yellow, red, and green triangles indicate the mutated amino acids that interact with Cm, DXC, both Cm and DXC, respectively.
