Figure 3

Determination of viral titers and genome copies in growth kinetics. DEF cells in 24-well plates were infected with 0.05 MOI of BAC-CHv, BAC-CHv-ΔUS3 or BAC-CHv-ΔUS3R. Samples were collected at the indicated time points, and viral titers and genome copies were determined. The data were presented as the mean ± standard deviation (SD) of three independent experiments. (a) Intracellular viral titers. (b) Supernatant viral titers. (c) Viral genome copies in total cells. (d) Viral titers in total cells. (e) The efficiency of infectious virion formation (Titers/Genome copies).