Table 3 Primer sequences for Real-time qPCR.
Target gene | Primer | Sequences 5′-3′ | Length | TM (oC) | Amplicon size (bp) | Accession No./ Reference |
|---|---|---|---|---|---|---|
IGF-I | F | GATCACGTGGCATTGTGGAC | 20 | 59.6 | 95 | DQ666526 |
| Â | R | AGCAGGCTTGCTAGTCTTGG | 20 | Â | Â | Â |
CYP7A1 | F | CATCTGTCAAGGCATTCGGC | 20 | 59.6 | 99 | KT827791 |
| Â | R | CCTCACCCTGCAAGGTCTTC | 20 | Â | Â | Â |
FASN | F | ATCCCTCTTTGCCACTGTTG | 20 | 57.5 | 121 | Yu et al.29 |
| Â | R | GAGGTGATGTTGCTCGCATA | 20 | Â | Â | Â |
Cu/Zn-SOD | F | CCACAGAAACTTACGCGACA | 20 | 58.5 | 100 | FJ030929 |
| Â | R | AAATAAACGGTCCCGGTGGT | 20 | Â | Â | Â |
GPx-4 | F | AGGTTTACGCATCCTTGCCT | 20 | 59.7 | 92 | AY309440 |
| Â | R | TGTAGGAATGGGCAAACTGCT | 21 | Â | Â | Â |
TNF-α | F | CTTCGTCTACAGCCAGGCATCG | 22 | 63.0 | 161 | Yu et al.29 |
| Â | R | TTTGGCACACCGACCTCACC | 20 | Â | Â | Â |
TGFβ1 | F | GCTCAAAGAGAGCGAGGATG | 20 | 59.0 | 118 | Yu et al.29 |
| Â | R | TCCTCTACCATTCGCAATCC | 20 | Â | Â | Â |
β-actin | F | ATCGCCGCACTGGTTGTTGAC | 21 | 60.0 | 336 | Chen et al.23 |
| Â | R | CCTGTTGGCTTTGGGGTTC | 19 | Â | Â | Â |
