Figure 4

Performance measurement of separating SNPs and artefacts using p-value by. a Receiver Operating Characteristics (ROC) curve. ROC curve based on p-values with false positive rate (FPR) on the x-axis and true positive rate (TPR) on the y-axis. The area under the curve (AUC) equals 0.9906 (99.06%), which means it has near optimal measure of separability. The black dashed line marks the cut-offs for \(\,{p}_{s}=0.05\). The theoretical optimal threshold for a p-value to distinguish between true SNPs and sequencing errors based on the ROC curve was calculated as \({p}_{g}=0.18\) and is shown as red dashed line