Figure 4
From: Chloroplast development at low temperature requires the pseudouridine synthase gene TCD3 in rice
Transcript levels of TCD3 in various tissues (A) and at different temperatures (B) determined by RT-PCR analysis; YR, young-seedling roots; ST, young-seedling stems; YL, young-seedling leaves; SL, the second leaf from the top; FL, flag leaf at heading; PN, young panicles. OsActin was used as a control (28 cycles for OsActin, 35 cycles for TCD3; Transcript levels (B) of TCD3 in WT and tcd3 at the 3-leaf stage at 20° and 32°, OsActin was used as a control for qPCR. Data are means ± SD (n = 3). Asterisks indicate statistically significant difference compared with WT. **P < 0.01 by Student’s t-test.
