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Figure 1

From: Antitumor effect of a WEE1 inhibitor and potentiation of olaparib sensitivity by DNA damage response modulation in triple-negative breast cancer

Figure 1

Anti-proliferative effect of AZD1775 in TNBC cells. (a) The different anti-proliferative effects of AZD1775 in TNBC cells. Growth inhibitions were measured using an MTT assay. Cells were treated with increasing doses of AZD1775 for 5 d. Percentages of surviving cells are presented with ±SE bars (n = 3). (b) p53 protein expression levels in TNBC cells increased with IC50 values. (c) AZD1775 treatment increased sub-G1 populations. Cellular DNA contents were measured by flow cytometry after PI staining. Sub-G1 proportions are shown in the bar graph. Bars represent ±SDs (n = 3). ***P < 0.001. (d) AZD1775 induced apoptosis in MDA-MB-231 cells. MDA-MB-231 and MDA-MB-468 cells were treated with AZD1775 at 350 nmol/L for 5 d, and annexin-V PI staining was performed to identify apoptotic cells. Bars represent ±SDs (n = 3). ***P < 0.001. (e) Caspase-dependent apoptosis induced by AZD1775. After 5 d of treatment with AZD1775 350 nmol/L, MDA-MB-231, and MDA-MB-468 cells were analyzed by Western blotting and probed with anti-PARP, caspase-3, and α-tubulin antibody. We used several gels to examine protein expression. But the results were all derived the same experiment, and the gels and blots were processed in parallel.

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