Figure 2
From: WNT and inflammatory signaling distinguish human Fallopian tube epithelial cell populations
Organoid assays from Fallopian tube epithelial cells isolated by ALDH activity. (A) Schematic describing experimental strategy. Epithelial cells from cryopreserved proximal and distal region Fallopian tube fragments were isolated by FACS on the basis of ALDH activity. (B-C) Representative FACS data. (B) Gates for proximal EpCAM+/ALDH− cells (blue rectangle) and EpCAM+/ALDH+ cells (red rectangle). Grey and black dots indicate EpCAM isotype control cells and cells treated with an ALDH inhibitor (DEAB), respectively. (C) Gates for distal EpCAM+/ALDH− cells (blue rectangle) and EpCAM+/ALDH+ cells (red rectangle). Grey and black dots indicate EpCAM isotype control cells and cells treated with an ALDH inhibitor (DEAB), respectively. (D) Organoid formation by EpCAM+ ALDH+ and EpCAM+ ALDH− cells isolated from proximal (purple markers) and distal (dark green markers) regions of Fallopian tubes.
