Figure 4
From: The Role of Bone Morphogenetic Protein Signaling in Non-Alcoholic Fatty Liver Disease
BMP2 increases DGAT2 expression and activity. Treatment of HepG2 cells with BMP2 resulted in increased expression of DGAT2 (diacylglycerol O-acyltransferase 2) mRNA (A, six replicates per group) as well as increased DGAT2 protein levels at 24hrs (B). In parallel experiments, DGAT activity was assessed by quantification of fluorescently-labeled triglyceride production in HepG2 cells treated with BMP2 or control at 24hrs with DGAT2 overexpression and control plasmid as additional controls (C, three replicates per group). Comparisons were made using the two-tailed Student’s t test.
