Figure 1

Tumor visualization in vivo in (A) non-cancerous and (B) cancerous larvae characterized by GFP-labeled cells (in white) in the eye-antennal disc region. (C) Fold induction of the three immune genes in cancerous larvae (yellow; n = 10 pools) without infection relative to non-cancerous larvae (dark blue; n = 10 pools). The error bars represent standard deviation (SD) of two independent experiments (***p < 0.001; **p < 0.01; *p < 0.05; Sidak’s test). Effect of infection by the bacterium Pectobacterium carotovorum carotovorum (Pcc, red; n = 96 total) and the fungi Beauvaria bassiana (Bb, orange; n = 102 total) compared to uninfected flies (blue; n = 97 total) on individual tumor size with a low intensity threshold of detection (S2 threshold: 75% of maximum intensity value) in three independent experiments. The error bars represent standard deviation (***p < 0.001; **p < 0.01; *p < 0.05; NS > 0.05; Tukey’s test). (E) Impact of body size, approximated by mouth hook size, on tumor size depending on infectious treatment (n = 20 by infection group; error bars are SD).