Figure 5

CaMKII and phosphorylated-CaMKII expression in cells co-transfected with WT or p.P888L SAP97. (a) WB images and their corresponding stain-free gels showing CaMKII expression (arrows) in CHO cells transfected with Kv4.3 channels alone or in the presence of WT or p.P888L SAP97. (b) Mean densitometric analysis of CaMKII levels normalized to total protein (n = 4). (c) (Top) WB images of phosphorylated-CaMKII expression in CHO cells co-transfected with Kv4.3 channels and WT or p.P888L SAP97. Na⁺-K⁺ ATPase was used as a loading control (bottom). (d) Mean densitometric analysis of phosphorylated-CaMKII levels normalized to the Na⁺-K⁺ ATPase density (n = 4). *P < 0.05 vs Kv4.3 and WT SAP97.