Figure 7
WT and p.P888L SAP97 similarly increased INa density. (a,b) Traces (a) and density-voltage relationships (b) of the INa recorded in Sham, WT and p.P888L SAP97 mouse cardiomyocytes. (c) WB image and its corresponding stain-free gel showing Nav1.5 (arrow) expression in ventricular tissue from Sham, WT and p.P888L SAP97 mice. (d) Mean densitometric analysis of Nav1.5 levels normalized to the total protein. (e) Inactivation and activation curves for INa recorded in cardiomyocytes from the three mouse groups. Continuous lines represent the Boltzmann fit of datapoints. (f) Time course of INa reactivation measured in cardiomyocytes from the three mouse groups. In b, e and f each point represents the mean ± SEM of “n” experiments/cardiomyocytes from five mice of each group. *P < 0.05 vs Sham mice.
