Figure 3 | Scientific Reports

Figure 3

From: Effects of novel non-thermal atmospheric plasma treatment of titanium on physical and biological improvements and in vivo osseointegration in rats

Figure 3

Biological effects of NTAP-Ti seeded with MC3T3-E1 cells. (A) SEM morphology of MC3T3-E1 cells on titanium surfaces at 12 h: (a) Ti, (b) NTAP-Ti. (B) Fluorescence microscopy images of MC3T3-E1 cells on titanium surfaces at 12 and 24 h: (a, c) Ti, (b, d) NTAP-Ti. Spreading area (C) and perimeter (D) of adherent MC3T3-E1 cells cultured on the titanium surfaces for 12 and 24 h. (E) Cell adhesion and proliferation of MC3T3-E1 cells on titanium surfaces after 3 h, 1 day, 3 days and 5 days of culture. Data are shown as mean ± SD; n = 6 specimens/group. Values with dissimilar letters are significantly different (P < 0.05).

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