Figure 4

VAMP1 and VAMP2 co-localize with endogenous DCV markers. (A) Hippocampal neuron (DIV 11) infected with TI-VAMP1 (DIV 2) and stained for Synapthophysin (Syph, magenta), VAMP2 (green) or TI-VAMP1 (blue). Lower panels are a zoom of the upper panel. Arrowhead: VAMP1-positive punctum, arrow: VAMP2 and Syph co-localization, double arrow: VAMP1, VAMP2 and Syph co-localization. (B) Pearson's coefficient of Syph and VAMP1 or VAMP2, and of VAMP1 and VAMP2. One-way ANOVA with post hoc Tukey's test: Syph-VAMP2 vs syph-VAMP1/VAMP2-VAMP1: p < 0.0001 (***), rest is non-significant (ns). (C) Mander's coefficient of the co-localisation of Syph, VAMP1 and VAMP2. One-way ANOVA with post hoc Tukey's test: Syph in VAMP2/VAMP2 in Syph vs Syph in VAMP1/VAMP1 in Syph and vs VAMP2 in VAMP1/VAMP1 in VAMP2, p = 0.0001 (***), rest is non-significant (ns). (D) Hippocampal neuron (DIV 11) stained for VAMP2 (green) and secretogranin II (SCG2, magenta). Lower panels indicate the zoom of upper panels. Arrowhead: SCG2 punctum, arrow: VAMP2 punctum, double arrow: VAMP2 and SCG2 co-localization. (E) Hippocampal neuron (DIV 11) stained for VAMP1 (green) and BDNF (magenta). Lower panels indicate the zoom of upper panels. Arrowhead: BDNF punctum, arrow: VAMP1 punctum, double arrow: VAMP1 and BDNF co-localization. (F) Pearson's coefficient of VAMP2 and SCG2, VAMP1 and BDNF, and VAMP2 and Syph. One-way ANOVA with post hoc Tukey's test: VAMP2-SCG2/VAMP1-BDNF vs VAMP2-Syph: p < 0.0001 (***), VAMP2-SCG2 vs VAMP1-BDNF: p = 0.69 (ns). (G) Mander's coefficient of the co-localization of VAMP2 and SCG2, VAMP1 and BDNF, and VAMP2 and Syph. One-way ANOVA with post hoc Tukey’s test: VAMP2 in SCG2/SCG2 in VAMP2 vs Syph in VAMP2/VAMP2 in Syph and VAMP1 in BDNF/BDNF in VAMP1 vs Syph in VAMP2/VAMP2 in Syph: p < 0.0001 (***) rest is non-significant (ns). Detailed statistics are shown in Supplementary Table S1.