Figure 5 | Scientific Reports

Figure 5

From: Tetanus insensitive VAMP2 differentially restores synaptic and dense core vesicle fusion in tetanus neurotoxin treated neurons

Figure 5

TI-VAMP1 and TI-VAMP2 co-travel with DCVs. (A) Representative kymographs of neurons co-infected (at DIV 4–6) with TI-VAMP1 and NPY-mCherry (left), or TI-VAMP2 and NPY-mCherry (right), imaged at DIV 11–13. VAMP kymographs in green, NPY kymographs in magenta. The bottom panels are graphic representations of the kymographs to show the quantification of the tracks: VAMP only (green), NPY only (magenta) or co-trafficking/localization (black). (B) Percentage moving (diagonal line in kymograph) TI-VAMP1 (n = 16, N = 2) TI-VAMP2 (n = 14, N = 2), and NPY (n = 30, N = 2) puncta. 1-way ANOVA with post-hoc Tukey’s test: VAMP2 vs VAMP1/NPY: p = 0.001 (***). (C) Percentage of moving NPY puncta with VAMP1 and VAMP2, and moving VAMP1 and VAMP2 puncta with NPY. 1-way ANOVA: p = 0.48 non-significant (ns). Bars represent mean + SEM. Detailed statistics are shown in Supplementary Table S1.

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