Figure 1

SDS-PAGE analysis of proteins expressed in transformed E. coli. Lane M Protein marker. Lane 1 Vector control expression in BL21 cultured for 4Â h with IPTG. Lane 2 and 3 Supernatant and precipitation of recombinant E. coli expressing Mce1C proteins cultured for 4Â h with 0.2Â mmol/l IPTG, respectively. Lane 4 and 5 Supernatant and precipitation of recombinant E. coli expressing Mce1D proteins cultured for 4Â h with 0.2Â mmol/l IPTG, respectively. Lane 6 purified Mce1D protein.