Figure 2 | Scientific Reports

Figure 2

From: Mce1C and Mce1D facilitate N. farcinica invasion of host cells and suppress immune responses by inhibiting innate signaling pathways

Figure 2

Mce1C and Mce1D protein can be recognized by antisera from mice and rabbits infected with N. farcinica. The antigenicity of Mce1C and Mce1D protein was analyzed using Western blotting. Lane M Protein marker. Lane 1 Protein from vector control E. coli induced for 4 h with 0.2 mmol/l IPTG. Lane 2 Protein from recombinant E. coli expressing Mce1C protein induced for 4 h with 0.2 mmol/l IPTG. Lane 3 Protein from recombinant E. coli expressing Mce1D protein induced for 4 h with 0.2 mmol/l IPTG. (a) Antisera from BALB/c mice infected with N. farcinica; (b) Antisera from BALB/c mice infected with normal saline; (c) Antisera from New Zealand White rabbits infected with N. farcinica; (d) Antisera from rabbits infected with normal saline.

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