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Figure 3

From: Photodynamic inactivation of Leishmania braziliensis doubly sensitized with uroporphyrin and diamino-phthalocyanine activates effector functions of macrophages in vitro

Figure 3

Uptake of photo-inactivated porphyric L. braziliensis by primary macrophages. Bone marrow-derived macrophages (BMDM) were co-cultured with L. braziliensis (Lb) transfectants (grey bar), those rendered porphyric by ALA-exposure (black bar) and those photo-inactivated by exposure of porphyric Lb to UV (+ L) for 30 min (white bar) at a host-parasite ratio of 1:10. Samples were taken at 6 h and 24 h for microscopy to assess: (A) the percentage of BMDM containing L. braziliensis; and (B) the number of Leishmania per 200 macrophages. Data are presented as mean ± S.D. from a representative experiment performed in quadruplicate, *p < 0.05. (C) Representative photomicrographs of (A, B). Arrow points to intracellular Lb.

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