Figure 6
From: Orexin system is expressed in avian liver and regulates hepatic lipogenesis via ERK1/2 activation
Orexin activates ERK1/2 signaling cascade in LMH cells. Orexin administration increased p-ERK1/2Thr202/Tyr204 levels, but not that of p-P38Thr180/Tyr182 or p-JNK1/2Thr183/Tyr185 in LMH cells as demonstrated by Western blot analyses (a–c). The relative expression of MAPK genes was measured by qPCR (d,e). Overexpression of ERK1 or ERK2 increased p-ERK1/2Thr202/Tyr204, activated ACC and increased ME protein levels (f,g) as well as the mRNA abundances of ACC, FASN, ME, ACLY and SREBP-1 (h). Data are presented as mean ± SEM (n = 6/group). *Indicates significant difference from controls at P < 0.05. ACC acetyl-CoA carboxylase, ACLY ATP citrate lyase, ERK extracellular signal-regulated kinase, FASN fatty acid synthase, JNK c-Jun N-terminal kinase, ME malic enzyme, P38 P38 mitogen-activated protein kinase, SREBP-1 sterol regulatory element-binding protein 1. Full-length blots/gels are presented in Supplementary Fig. S1.
