Table 3 Key resources.
Product name | Company | Cat # |
|---|---|---|
Antibodies | ||
β-actin | Santa Cruz | SC-47778 |
P-Histone H2A.X | Cell Signaling | 2577 |
Cleaved caspase-3 | Cell Signaling | 9661 |
PARP1 | Cell Signaling | 9532 |
PAR polymers | Trevigen | 4335-MC-100-AC |
Amino acid-deficient culture medium | ||
DME high glucose minus arg, leu, lys, met | Athenaes | 0420 |
DME low glucose minus arg, gln, leu, lys | Sigma | D9443 |
RPMI minus arg, lys | Thermo-Fisher | 89984 |
Radioactive chemicals | ||
[8-14C]Hypoxanthine | Moravek, Inc | MC-147 |
[1-14C]D-Glucose | Moravek, Inc | MC228W |
[14C]Formic acid sodium salt | Moravek, Inc | MC173 |
[Methyl-3H]Thymidine | Moravek, Inc | MT6039 |
Reagents | ||
Cell Titer-Glo 2.0 Assay | Promega | G9248 |
Cis-Diammineplatinum(II) dichloride | Millipore-Sigma | P-4394 |
Human epidermal growth factor | Millipore-Sigma | E9644 |
Lipofectamine 2000 | Thermo-Fisher | 11668019 |
5-Phospho-D-ribose 1-diphosphate, pentasodium salt | Millipore-Sigma | P-8296 |
D-Ribose 5-phosphate, disodium salt | Millipore-Sigma | 83875 |
Thiazolyl blue tetrazolium bromide | Millipore-Sigma | M2128 |
Rodent chow | ||
Amino acid-defined diet | Teklad | 86529 |
Amino acid-defined diet minus lysine | Teklad | 99386 |
